Fig. 4

AGK increases the population of EOC CSC and induces chemoresistant in vitro. A A real-time PCR analysis of the expression of pluripotency-associated markers, ALDH1, Sox2, Oct4, Nanog and Lin28 in the indicated cells. B Representative images (left panel) of spheres formed by the indicated cells. Histograms (right panel) showed the mean number of spheres formed by the indicated cells. C Single-AGK-overexpressing and control cells were performed for their sphere-forming potential in 96-well plates. Tertiary and quaternary spheres were generated in cells dissociated from 1 previous individual generation sphere in each 96-well plate. Data was compiled from 3 independent biologic experiments. D Hoechst-stained side population (SP) assay showed that the overexpression of AGK was promoted, whereas AGK knockdown attenuated the SP cells in the indicated cells. E Histograms show the mean percent of SP⁺ cell numbers. F Flow cytometry analysis of CD44⁺ cells (left panel). Histograms (right panel) show the mean percent of CD44+ cells