Fig. 5

c-Jun and c-Fos upregulate NEAT1 transcription. A Heatmap showing the list of upregulated lncRNAs in the RNA sequencing data between LacZ- and RPRD1B-overexpressed HGC27 cells, according to q value (qualified P value). NEAT1 ranked first with the most significance (q = 9.38E-49). The mRNA expression level of NEAT1 were confirmed upregulation in RPRD1B-overexpressing HGC27 and SGC7901 cells. B NEAT1 overexpression was confirmed in our cohort of patients with GC (n = 33). Overall survival curves for patients with GC in TCGA cohort according to the expression status of NEAT1 (n = 876). C Potential transcription factors of NEAT1 were predicted in ALGGEN website. First 15 transcriptional factors including c-Jun, c-Fos and AP1 were listed. D The luciferase activity of NEAT1 was increased following c-Jun/c-Fos transfection in HGC27 and SGC7901 cells. The effect was diminished by SR11302. E, F RNA-FISH and qRT–PCR showed that NEAT1 was upregulated in RPRD1B-overexpressing HGC27 and SGC7901 cells. The effect was diminished by SR11302. G, H NEAT1 was downregulated in AGS and BGC823 RPRD1B-silenced cells and rescued by c-Jun and c-Fos. Scale bar, 20 μm. Data are presented as the means ± SD of three independent experiments. (***, P < 0.001)