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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Suppression of VEGFD expression by S-nitrosylation promotes the development of lung adenocarcinoma

Fig. 2

NO suppresses VEGFD in protein level. A NO levels in lungs with urethane-induced tumors, quantification was based on 3 samples of each group. B NO levels in LLC cell xenografts, quantification was based on 3 samples of each group. C The mRNA level of ADH5 in lung adenocarcinoma extracted from the database of TCGA. D The Protein level of GSNOR in lung adenocarcinoma extracted from the database of CPTAC. E-F Western blot analysis of VEGFD protein levels in carcinogen urethane–induced and LLC xenograft tumors, and their quantification. Numerical data were expressed as mean ± SD (each n = 3). G-H Western blot analyses in NCI-H1975 cells treated with DTT, L-NAME, GSNO or SNP at the indicated concentrations for 24 h. K-L GSNO was administered to eight-week-old C57/BL6 female mice at the doses of 0.5 and 1.5 mg/kg (blank control) by single intratracheal instillation, lungs were used for sectioning. IHC for VEGFD, and their quantification. Numerical data were expressed as mean ± SD (each n = 3). Data in (G), (H), (I), and (J) are representative of three independent experiments, and data in (A), (B), (E), and (F) represent the mean ± SEM of triplicate samples. *P < 0.05, **P < 0.01, Student’s t test

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