Fig. 4

VEGFD is S-nitrosylated at Cys277. A-C HEK-293T cells were transfected with wild type VEGFD (WT) or its S-nitrosylation-deficient mutants, cultured for 24 h, and then treated with or without 50 µM GSNO for further 24 h, Acyl-biotinyl exchange (ABE) and Western blotting analysis VEGFD. D-E HEK-293T cells were transfected with WT or VEGFD(C277S) mutant, cultured for 24 h, and then treated with 5 µg/µL CHX or 5µM MG132 at different times for the indicated times, VEGFD were detected by western blot analysis. F Structural modeling of VEGFD (residues 273–315) and the molecular contacts around Cys277. Data in (A), (B), (C), (D), and (E) are representative of three independent experiments