Fig. 1
CCDC6 protein levels contribute to the differential sensitivity to PARPi and PARGi in HGSOC. A-C PARP-inhibitor olaparib [at 1 μM (Ola 1 μM)] and PARG-inhibitor [at 1 μM (PARGi 1 μM)] sensitivity in HGSOC cells: clonogenic assays were carried out in Kuramochi (A), OVCAR3 (B) and OV-90 (C) ovarian cancer cells silenced, upon transfection with short hairpin RNA for CCDC6 (ShCCDC6) or following treatment with P5091 [2.5 μM]. Scrambled shRNA (ShCtrl) or vehicle (DMSO) are used as controls. Cells were continuously exposed to DMSO, to 1 μM olaparib, or to 1 μM PARG-inhibitor PDD00017273. Colonies containing at least 30 cells were considered. The percentage of counted colonies were reported on the histograms on the right, for each cell line. Error bars indicate the measurement of the standard error mean, derived from three independent experiments. Statistical significance was verified by 2-tailed Student's t-test (* p < 0.05; ** p < 0.01 and *** p < 0.001)