Fig. 6

The YTHDC1-ANAX1 axis regulates the sensitivity of sunitinib in ccRCC. A, A498 and 786-O cells were transfected with indicated shRNAs for 72 h. Cells were treated with a serial dose of sunitinib for 24 h and harvested for CCK-8 assay. B, A498, 786-O and 786-O R (sunitinib resistance) cells were transfected with indicated plasmids for 72 h. Cells were treated with a serial dose of sunitinib for 24 h. Cells were harvested for CCK-8 assay. C and D, 786-O and A498 cells were transfected with indicated shRNAs for 72 h. Cells were treated with or without sunitinib (2 µM) and subjected to CCK-8 assay (C) and Annexin V-PI assay (D). Data presents as mean ± SD with three replicates. *, P < 0.05; ***, P < 0.001. E and F, 786-O and A498 cells were transfected with indicated plasmids for 24 h. Cells were treated with or without sunitinib (2 µM) and subjected to CCK-8 assay (E) and Annexin V-PI assay (F). Data presents as mean ± SD with three replicates. **, P < 0.01; ***, P < 0.001. G, A498 and 786-O cells were transfected with indicated shRNAs for 72 h. Cells were treated with a serial dose of sunitinib for 24 h and harvested for CCK-8 assay. H, A498 and 786-O cells were transfected with indicated constructs for 48 h. Cells were treated with a serial dose of sunitinib for 24 h and harvested for CCK-8 assay. I, A498 and 786-O cells were transfected with indicated constructs for 48 h. Cells were harvested for CCK-8 assay. Data presents as mean ± SD with three replicates. ***, P < 0.001. J, A498 and 786-O cells were transfected with indicated constructs for 72 h. Cells were harvested for CCK-8 assay. Data presents as mean ± SD with three replicates. *, P < 0.05; ***, P < 0.001. K-M, 786-O cells were transfected with indicated shRNAs for 72 h. After puromycin selection, cells were collected and subcutaneously injected into the nude mice. These mice were treated with or without sunitinib. The tumor mass and tumor growth curve were shown in panel L and panel M. Data presents as mean ± SD with six replicates. **, P < 0.05; ***, P < 0.001