Fig. 4
From: GRK3 is a poor prognosticator and serves as a therapeutic target in advanced gastric adenocarcinoma
GRK3 and YAP1 expression positively correlated in GAC tissues and GRK3 induced YAP1 expression in GAC cells. A The correlation between GRK3 and YAP1 in TCGA human STAD patients (n = 415). Pearson’s correlation test was used. P = 0.0058. B qPCR performed in tumor tissues and malignant ascites cells from 66 GAC cases in our cohort showed that GRK3 positively correlated with YAP1 in GAC and peritoneal metastasis. r = 0.5082, P < 0.001. C Representative images on IHC staining of YAP1 and GRK3 proteins showed that expression of YAP1 and GRK3 was positively associated within tumor tissues. D Chi-square test analysis indicated a significant positive association between GRK3 and YAP1 expression in the same cohort of TMAs. E The correlation of GRK3 expression with histological type was analyzed in TMAs. P < 0.01. F GRK3 and YAP1 expression was detected using co-immunofluorescent staining in 49 cases of malignant ascites cells; Representative images and quantification of GRK3lo/YAP1lo and GRK3hi/YAP1hi are shown. r = 0.8140; P < 0.0001. Scale bar: 25µm. G Overexpression GRK3 in KATO III cells dramatically increased YAP1 expression by qPCR; **P < 0.001; H Co-staining of YAP1 and GRK3 in KATO III cells with transfected with either GRK3 OE or mutant GRK3 cDNA compared to control cells; Scale bar:50µm. I&J Knockdown of GRK3 in GA0518 cells dramatically decreased YAP1 expression as shown by western blot (I) and qPCR (J) which correspond to the decrease in GRK3 level. K. Level of YAP1 and GRK3 was determined using western blot (upper panel) and qPCR (bottom panel) in GA0518 cells treated with LD2 at the dosage indicated. **P < 0.001