Fig. 6

CircARID1A and IGF2BP3 regulate the expression of SLC7A5 in GC cells. A Potential circARID1A-IGF2BP3 targets. B Expression of IFITM3 and SLC7A5 mRNA in SGC7901 following IGF2BP3 knockdown and in AGS cells following IGF2BP3 overexpression. C Expression of SLC7A5 protein in SGC7901 and BGC823 cells following IGF2BP3 knockdown. D Expression of SLC7A5 protein in AGS cells following IGF2BP3 overexpression. E–F Expression of SLC7A5 (E) mRNA and (F) protein in SGC7901 and BGC823 cells following circARID1A knockdown. G-H Expression of SLC7A5 (G) mRNA and (H) protein in AGS cells transfected with vector or IGF2BP3 and co-transfected with NC or circARID1A. I-J Expression of AKT, mTOR, p-AKT and p-mTOR in SGC7901 and BGC823 cells following (I) SLC7A5 knockdown and (J) following circARID1A knockdown. The P-values were calculated using the two-tailed Student’s t test or one way ANOVA. *P < 0.05