Fig. 3

Preparation and characterization of PSPM-NPs. A Strategies for preparing MUC12-targeted PSPM-NPs. B TEM mapping of PSPM-NPs. C EDS imaging for PSPM-NPs. D Zeta potentials of PSP and PSPM-NPs. E Elevated diameters of PSP and PSPM-NPs. F, G Agarose gel electrophoresis (F) and colloidal Coomassie images (G) of PSP and PSPM-NPs. H Images of PSPM-NPs-mediated cytophagocytosis of ACHN cells. I (c) Serum analysis of BUN, creatinine, ALT, and AST in mice after PSPM-NPs i.v. injection. J H&E staining of lung, liver, spleen, kidney and heart in mice treated with PSPM-NPs. Abbreviations: PSPM-NPs, Plasmid-SLERCC@PDA@MUC12 nanoparticles; PSP, Plasmid-SLERCC@PDA; EDS, Energy dispersive X-ray spectrometry; TEM, Transmission electron microscopy; BUN, Blood urea nitrogen; ALT, Alanine aminotransferase; AST, Aspartate aminotransferase