Fig. 5

DNMT3A-mediated SLERCC promoter hypermethylation results in the downregulation of SLERCC in RCC cells. A CpG islands of SLERCC as predicted by MethPrimer 2.0. B Relationship of DNMT1, DNMT3A, and DNMT3B with SLERCC in TCGA dataset. C-E Expression of SLERCC in 786-O and ACHN cells after 5-AZA treatment. F, G Growth curves of 786-O cells (F) and ACHN cells (G) were measured after transfection with indicated vectors by CCK-8 assays. H, I IHC staining for DNMT3A in stages I and IV, primary carcinoma, and sternal metastatic tissues. J Relationship between DNMT3A and SLERCC in the TCGA dataset. K BSP assay detects DNA methylation levels of DNMT3A at CpG islands in the SLERCC promoter region in ACHN cells. L Relative expression of SLERCC confirmed by qRT-PCR in 786-O and ACHN cell lines transfected with si-DNMT3A, si-NC, vector or DNMT3A. (*p < 0.05, **p < 0.01, ***p < 0.001). Abbreviations: TCGA, The Cancer Genome Atlas; IHC, immunohistochemistry