Fig. 8

SLERCC directly interacts with UPF1 to inhibit RCC progression through the Wnt/β-catenin signaling pathway. A KEGG enrichment analysis for differentially expressed genes. B, C Relative expression of UPF1 as confirmed by qRT-PCR in 786-O and ACHN cell lines. D, G The rescue effects of UPF1 on the cell proliferation in SLERCC expressing 786-O and ACHN cells as assessed by CCK8 and EdU assays. H, J The rescue effect of UPF1 on the angiogenic capacity of SLERCC expressing 786-O and ACHN cells as assessed by the tube formation assay. I, K The rescue effect of UPF1 on the stemness of SLERCC in 786-O and ACHN cells as assessed by tumorsphere assay. L Western blotting to detect the changes in the expression of individual proteins after overexpressing SLERCC in 786-O cells. M Western blotting to detect changes in the expression of individual proteins after knocking down SLERCC in ACHN and Caki-1 cells. N Western blotting to detect the rescue effects of UPF1 on SLERCC-mediated changes in the expression of individual proteins in 786-O and ACHN cells. (**p < 0.01, ***p < 0.001). Abbreviations: RCC, Renal cell carcinoma