Fig. 3

Exogenous DJ-1 promotes metastasis of bystander ESCC cells by activating TGF-β1 pathway. A Representative IF images of the intracellular localization of exogenous DJ-1 (red) tagged by His-tag (green) in ESCC cells detected using immunofluorescence microscopy. Scale bar, 20 μm. B Heatmaps of 59 representative DEGs screened from RNAseq analysis. C KEGG pathway enrichment analyses showed top activated gene sets ordered by rich ratio and the TGF-β1 signaling pathway was enriched after DJ-1 stimulation. D A network of proteins enriched in the TGF-β1 signaling pathway and encoded by DEGs from RNAseq results. E Western blot results of prominent proteins correlated with the TGF-β1 signaling pathway in bystander ESCC cells co-cultured with irradiated cells or DJ-1. F-G Representative images and the statistical graph of transwell assays for bystander ESCC cells measured by LY2109761. Scale bars, 100 μm. ****p < 0.0001