Fig. 1
Schematical representation of 53X tribodies. A Scheme of constructs for 53X tribodies. SP, human interleukin (IL-2) signal peptide; VH_5T4, VL_5T4, amino acid sequences coding for the immunoglobulin heavy and light chain variable regions from Tb535H with specificity for human 5T4; CH1, CL, amino acid sequences coding for the human immunoglobulin heavy chain constant region 1 and the kappa light chain constant region, respectively; VH_CD3, VL_CD3, amino acid sequences coding for the variable heavy and light chain regions from humanized OKT3 building a scFv with specificity for human CD3; L1, L2, aminoacid sequences with flexible linker, GPGGGSPG, and GGGGSGGGGSGGGGS [(GGGS)3], respectively. VH_C, VL_C, amino acid sequences coding for the variable heavy and light chain from PD-1_1, PD-L1_1, 10_12, LAG3_1, scFvs with specificities for human PD-1, PD-L1 or LAG-3, respectively. The scFv from Palivizumab was used in an additional tribody for isotype control; 6xHis, amino acid sequences coding for a hexahistidine tag. B Scheme of assembled tribody proteins for Tb535H [(5T4)2 × CD3], 53D [5T4 x CD3 x PD-1], 53L1 [5T4 x CD3 x PD-L1], 53L10 [5T4 x CD3 x PD-L1], 53G [5T4 x CD3 x LAG-3] and 53P [5T4 x CD3 x isotype control], respectively