Fig. 2

Binding affinity of parental Tb535H and novel 53X tribodies to recombinant 5T4 protein by ELISA. A Binding curves by ELISA assays of tribodies (0–500 nM) to immobilized recombinant human 5T4 protein. Binding values were reported as the mean of determinations obtained in three independent experiments. B Table reporting the binding affinity or EC50 (nM) values for 5T4 of each indicated construct. Standard Deviations were ≤ 3—10%