Fig. 2
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13046-022-02474-3/MediaObjects/13046_2022_2474_Fig2_HTML.png)
Binding affinity of parental Tb535H and novel 53X tribodies to recombinant 5T4 protein by ELISA. A Binding curves by ELISA assays of tribodies (0–500 nM) to immobilized recombinant human 5T4 protein. Binding values were reported as the mean of determinations obtained in three independent experiments. B Table reporting the binding affinity or EC50 (nM) values for 5T4 of each indicated construct. Standard Deviations were ≤ 3—10%