Fig. 4

Binding affinity of indicated tribodies for recombinant PD-L1 or PD-1 proteins by ELISA. A Binding curves obtained by ELISA assays of 53L1 and 53L10 tribodies (0 – 500 nM) on immobilized recombinant human PD-L1 proteins. B Binding curves obtained by ELISA of 53D tribody (0–500 nM) tested on immobilized recombinant human PD-1 protein. Binding values were reported as the mean of at least three determinations obtained in three independent experiments. Standard Deviations were ≤ 3—10%