Novel tri-specific tribodies induce strong T cell activation and anti-tumor effects in vitro and in vivo

Table 1 List of tribodies generated in the study

Name of product	Structure		Fab	scFv
Tb535H	(Fab) 5T4 – (scFv) CD3 – (scFv) 5T4	H – chain derivative	hu5T4	huOKT3
Tb535H	(Fab) 5T4 – (scFv) CD3 – (scFv) 5T4	L – chain derivative	hu5T4	hu5T4
53L1	(Fab) 5T4 – (scFv) CD3 – (scFv) PD-L1	H – chain derivative	hu5T4	huOKT3
53L1	(Fab) 5T4 – (scFv) CD3 – (scFv) PD-L1	L – chain derivative	hu5T4	PD-L1_1
53L10	(Fab) 5T4 – (scFv) CD3 – (scFv) PD-L1	H – chain derivative	hu5T4	huOKT3
53L10	(Fab) 5T4 – (scFv) CD3 – (scFv) PD-L1	L – chain derivative	hu5T4	10_12
53D	(Fab) 5T4 – (scFv) CD3 – (scFv) PD-1	H – chain derivative	hu5T4	huOKT3
53D	(Fab) 5T4 – (scFv) CD3 – (scFv) PD-1	L – chain derivative	hu5T4	PD-1_1
53G	(Fab) 5T4 – (scFv) CD3 – (scFv) LAG-3	H – chain derivative	hu5T4	huOKT3
53G	(Fab) 5T4 – (scFv) CD3 – (scFv) LAG-3	L – chain derivative	hu5T4	LAG-3_1
53P	(Fab) 5T4 – (scFv) CD3 – (scFv) Palivizumab	H – chain derivative	hu5T4	huOKT3
53P	(Fab) 5T4 – (scFv) CD3 – (scFv) Palivizumab	L – chain derivative	hu5T4	Palivizumab

Amino acidic sequences of Tb535H were described in WO2016097408 (A1). Amino acidic sequences of PD-1_1, PD-L1_1, 10_12, LAG-3_1 were described in WO2019180201A2

ISSN: 1756-9966