Fig. 3
From: Peptide-based PROTAC degrader of FOXM1 suppresses cancer and decreases GLUT1 and PD-L1 expression
FOXM1-PROTAC induces FOXM1 protein degradation. A Structure of FOXM1-PROTAC. B Viability of cells, treated with FIP-1 and FOXM1-PROTAC for 24 h, were performed using CCK-8, and the results showed that FOXM1-PROTAC has a better inhibitory effect on cells. C, Western blotting examination for FOXM1 of HepG2 cells treated with 20 μM FOXM1-PROTAC for different time (0, 3, 6, 12, 24, 48 h). D Western blotting test for FOXM1 of HepG2 cells treated for 24 h with increasing concentrations of FOXM1-PROTAC (0, 2, 5, 10, 20, 30, 50 μM). E Expression of FOXM1 in HepG2 and MDA-MB-231 cells treated with 20 μM FDI-6, FIP-1 and FOXM1-PROTAC for 24 h. F Detection of FOXM1 mRNA by Real-time PCR in HepG2 and MDA-MB-231 cells treated with 20 μM FOXM1-PROTAC for 24 h. G HepG2, A549, MDA-MB-231, HCT116 cells viability, treated with 20 μM FOXM1-PROTAC for 24 h respectively, were performed using CCK-8