Fig. 4
FAM83H-AS1 regulates β-catenin expression and stabilizes FAM83H mRNA in PDAC cells. A The unidimensional location diagram of FAM83H-AS1 and the nearby gene FAM83H in the UCSC database. B, C The positive correlation between FAM83H-AS1 and FAM83H expression in PDAC tissues from the Tongji Hospital cohort (B) and TCGA cohort (C) was confirmed by Spearman’s correlation analysis. D, E Western blotting and IF staining were used to assess the expression of FAM83H in PDAC cells upon FAM83H-AS1 overexpression and knockdown; scale bar: 50 μm. F FAM83H-AS1 did not affect FAM83H protein stability, as shown by determining the effect of FAM83H-AS1 overexpression on the FAM83H degradation rate after addition of cycloheximide (100 μg/mL). G RT-qPCR results showed that FAM83H-AS1 decreased FAM83H mRNA degradation (right panel), while it did not affect GAPDH degradation (left panel) after addition of the RNA synthesis inhibitor actinomycin D (10 μg/mL). H Western blot results showed changes in the expression of the key proteins involved in reported mainstream signaling pathways upon FAM83H-AS1 overexpression and knockdown. *P < 0.05