Fig. 8
FAM83H-AS1 regulates PDAC progression via FAM83H/Wnt/β-catenin signaling. PDAC cells were treated with NC, LV-FAM83H-AS1, LV-FAM83H-AS1 + sh-FAM83H#1, LV-FAM83H-AS1 + sh-FAM83H#2 and LV-FAM83H-AS1 + VAX-939. A, B Transwell and wound-healing assays were conducted to assess the invasion and migration of the indicated groups of SW 1990 cells; scale bar: 100 μm. C Colony formation assays were used to evaluate the proliferation of the indicated groups of SW 1990 cells. D Western blotting was conducted to determine the expression of FAM83H, β-catenin, N-cadherin, Vimentin, CD44 and MMP2. E HEK293T cells were cotransfected with HA-β-catenin, FLAG-FAM83H, sh-FAM83H-AS1 and MYC-Ub and then treated with 10 μM MG132 for 6 h. Polyubiquitinated proteins were immunoprecipitated with anti-HA beads, followed by immunoblotting with antibodies against MYC and HA. *P < 0.05, **P < 0.01 and ***P < 0.001