Fig. 6

CircPAK1 mediates oncogenic effects on HCC through inactivating Hippo signaling pathway. A Pathway enrichment analysis of differentially expressed genes in RNA-sequence data. B Levels of p-YAP (ser127), LATS1/2 and p-LATS1/2 were examined by western blotting in circPAK1 stable knockdown or overexpression cells. C The expression of YAP1 protein in nuclear and cytoplasmic component of circPAK1 stable knockdown or overexpression cells. D IF assay in stable circPAK1-knockdown LM3 cells or stable circPAK1-overexpressed Hep-3B cells, scale bar, 20 μm. E The level of downstream target gene of YAP, CTGF and CYR61, were determined by qRT-PCR. *p < 0.05; **p < 0.01; ***p < 0.001. Data were shown as mean ± SEM