Fig. 1

GC-derived exosomes promote liver metastasis. A. Representative images of exosomes from MKN45 and MKN45-HL cells at TEM, Scale bar = 100 nm. B, C. Purified MKN45 and MKN45-HL exosomes were analyzed by NanoSight. D. Western blot analysis of exosome markers (TSG101 and CD81) in exosomes and lysates from MKN45 and MKN45-HL cells. E. Schematic representation of the establishment process of the exosome-educated GC-LM model. F. Representative in vivo imaging system (IVIS) results of mice injected with luciferase-labeled MKN45 cells into the spleen after educated with PBS, MKN45 exosomes, or MIN45-HL exosomes, respectively. Results of quantified values of bioluminescence imaging signals are expressed as mean ± standard deviation (n = 6). G-I. Effect of GC-derived exosomes on liver metastasis in mice. The representative photographs of liver metastasis, liver weight, and H&E staining were shown. The scale bars are 1.0 cm and 500 μm, respectively. J. Representative images of CD31 immunohistochemical staining of LM tissues from mice with exosome education, Scale bar = 100 μm. Data are shown as mean ± standard deviation of 3 independent experiments, and statistical significance was determined using one-way ANOVA test (*P < 0.05, **P < 0.01, ***P < 0.001)