Fig. 2

Establishment of SE-driven core transcriptional regulatory network of G3-MB. a Workflow of identifying vital SE-associated gene signature (vSE) of G3-MB. b Summary of the results from the gene expression and tumor dependency analyses of the 14 vSE genes identified in a. c-f RT-qPCR analysis of the 12 selected vSE genes in MB002 (c) or D425 (e) cells with each of these genes knocked down by two separate shRNAs individually. Cell viability of MB002 (d) or D425 (f) cells under above-mentioned conditions were measured at Day 0/2/4 post puromycin selection. g RT-qPCR analysis of vSE genes in MB002 or D425 cells when MYC, OTX2 and CRX were knocked down by shRNA individually. The mean relative expression levels are shown. h Schematic diagram of the SE-associated TF-effector-gene regulatory axis in the identified SE-driven core transcriptional regulatory network of G3-MB. All RT-qPCR and cell viability assays were performed in triplicate and the data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA (c-f) and two-tailed unpaired t test (g), respectively