Fig. 1

mTORC1 upregulates the expression of miR-130b-3p. A Venn diagram analysis of differentially expressed miRNAs in two datasets. Dataset 1 represents differentially expressed miRNAs between Tsc2 − / − and Tsc2 + / + MEFs. Dataset 2 represents dysregulated serum miRNAs between TSC patients and healthy controls. B Tsc2 + / + , Tsc2 − / − , rapamycin (20 nM, 24 h) treated Tsc2 − / − , Tsc1 + / + , Tsc1 − / − and rapamycin (20 nM, 24 h) treated Tsc1 − / − MEFs. C Tsc2 − / − or Tsc1 − / − MEFs were infected with lentiviruses harboring vectors encoding Raptor shRNA (shRaptor), Rictor (shRictor) or the control scrambled shRNA (shSc). D Tsc2 + / + or Tsc1 + / + MEFs were treated with or without MHY-1485 (1 or 5 μM) for 24 h. B-D Cell lysates were subjected to immunoblotting with the indicated antibodies (up panels); miR-130b-3p level was analyzed by qRT-PCR (low panels). Data indicate mean ± SD of triplicate samples. ** P < 0.01; *** P < 0.001; **** P < 0.0001; n.s indicates no significant difference