Fig. 5

miR-130b-3p promotes angiogenesis via targeting MBNL1. A-D Tsc2 − / − MEFs were infected with lentiviruses harboring control vector (Lv) or MBNL1 overexpression constructs (Lv-MBNL1). A The expression of MBNL1 was determined by western blot. B Cell culture supernatants from the cells were analyzed for VEGF-C by ELISA. (RU, relative unit). C and D The effect on angiogenesis of was detected by tube formation assay (C, scale bar, 50 μm) and CAM assay (D). E–G anti-miR-130b-3p-expressing NTC/T2 null cells were infected with lentivirus harboring shRNAs targeting MBNL1 (shMBNL1-1 and shMBNL1-2) or a control shRNA (shSc). E Cell lysates were subjected to immunoblotting with the indicated antibodies. F and G The angiogenic abilities of the indicated cells were detected by tube formation assay (F, scale bar, 50 μm) and CAM assay (G). Representative images (left panels) and quantifications (right panels). H–K The indicated cells were inoculated subcutaneously into nude mice (n = 5), and tumor growth was monitored. H Tumor pictures. Scale bar, 1 cm. I Tumor volumes, J Tumor weight. K Tumor tissues were subjected to HE and IHC staining. Scale bar, 50 μm. Data indicate mean ± SD of 3–5 replicates. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001