Fig. 1

Migration of breast tumour cells in the aerotactic assay. (A) Schematic representation of the aerotactic migration assay. (B) Radial aerotactic migration from the central cluster of untransformed MCF10A cells in a 96-well plate over 24 and 48 h. The graph on the right corresponds to the distribution of MCF10A cells from the centre of the well (in mm) at 0 h (yellow), 24 h (blue) and 48 h (carmine) after confinement (mean of three experiments). The Y-axis represents cell-density in arbitrary unit as described in the Methods section. (C) Classification of tumour cells relative to MCF10A according to their speed of migration in the aerotactic test. D5% is the minimum distance travelled by the fastest 5% cells (see Methods). (D) Representative images taken at 48 h corresponding to the aerotactic migration of T4, T5, T6, T7, T8, T11, T12, T13, T17, T18, T19 and T20 tumour cells subjected to the aerotactic assay. Cell distribution from the centre of the well at 0 h (yellow) and 48 h (carmine) is indicated as graphs (mean of three experiments). (See also Figure S1 for T9, T10, T15 and T16 cells). Y-axis scale is expressed in arbitrary unit. Scale bars, 0.5 mm