Fig. 6
From: EGFR-dependent aerotaxis is a common trait of breast tumour cells
Aerotaxis triggers invasion of tumour cells through extracellular matrix (Cultrex BME). (A) Aerotactic assay of untransformed MCF10A cells within the ECM (+ ECM) compared to the standard condition (-ECM). (B) Cell distribution from the centre of the cell cluster (in mm) of MCF10A at 0 h (carmine), 30 h (blue) or 60 h (yellow) in ECM and standard conditions. Y-axis scale is expressed in arbitrary unit. (C) Aerotactic assay of T6 tumour cells within the ECM compared to the standard condition (-ECM). Scale bars in A and C: 0.5 mm. (D) Cell distribution from the centre of the cell cluster (in mm) of T6 tumour cells at 0 h (carmine), 30 h (blue) or 60 h (yellow) in the ECM and standard conditions (mean of three independent experiments). Y-axis scale is expressed in arbitrary unit. (E) Magnified image of the aerotactic invasion of T6 tumour cells within the ECM at 18, 36 and 62 h after confinement. Scale bars, 250 μm