Fig. 1

LAMC2 expression in PDAC correlates with poorer outcome. a Boxplots illustrating differential expression of LAMC2 in PDAC tissue versus normal adjacent tissue using the indicated series of transcriptomic data. **** p < 0.0001. Statistical significance was assessed by Student's t-test. b Kaplan–Meier curves showing overall survival of PDAC patients, stratified according to the median value of LAMC2 expression. c Dimensional reduction plot (DimPlot) of stromal versus ductal (tumor) cells identified in PDAC primary tumors by scRNA-Seq. The clusters are color-coded based on cell types identified using known cell type-specific markers and are visualized using t-SNE. d Feature Plot for LAMC2 expression in multiple cell types identified in PDAC primary tumors by scRNA-Seq. The clusters are color-coded based on LAMC2 expression and are visualized using t-SNE. e Feature Plot for basal-like gene signature expression score in multiple cell types identified in PDAC primary tumors by scRNA-Seq. The clusters are color-coded based on LAMC2 expression and are visualized using t-SNE. f Representative images of IHC staining for LAMC2 (brown) in tissue sections from normal pancreas (P) and patients with PDAC tumors at G1, G2 and G3 grade. g H-score for LAMC2 expression. h qPCR analysis for LAMC2 expression in adherent cells. Data are normalized to GAPDH expression. i Western blot analysis of LAMC2 in adherent cells. Parallel β-ACTIN immunoblotting was performed. n \(\ge 3\)