Fig. 5

Potential receptor-ligand interactions between PDAC organoids and CAFs. A Number of potential ligand-receptor interactions between the different CAF and PDAC organoids cell clusters. B Potential receptor-ligand interactions between PDAC organoid and CAF cell clusters with a known or presumed role in EMT. Shown are only interactions where CAF cells present a ligand to the receptor expressed by PDAC organoid cells. C Immunofluorescence staining for CD44 (cyan) and HGF (magenta) of the parental tumor tissues of PDO/CAF lines 100, 107 and 112 confirms co-localization of a predicted receptor-ligand interaction in vivo. Single channels and composite are shown. DAPI stained nuclei are depicted in blue. Scale bar: 50 μm