Fig. 4

ARPC1B maintains the stability of IFI16 and HuR by direct binding. A ARPC1B-interacting proteins in GSC 267 cells were separated by SDS–PAGE for silver staining and mass spectrometry analysis. B The protein expression of IFI16 and HuR after ARPC1B knockdown with or without MG132 treatment (10 µM, 12 h). C Endogenous reciprocal Co-IP experiment testing the interaction between ARPC1B and IFI16 or HuR in GSC 267 and GSC 20 cells. D Co-IF staining showing the distribution of ARPC1B with IFI16 or HuR in GSC 267 cells. Scale bar, 5 μm. E The effect of ARPC1B knockdown on IFI16 and HuR protein levels in GSC 20 and GSC 267 cells treated with 100 μg/ml CHX for indicated times. F The ubiquitylation of IFI16 and HuR in GSC 20 and GSC 267 cells upon knockdown of ARPC1B. GSCs were pretreated with MG132 (10 µM) for 6 h before cell lysates were collected