Fig. 5

Gene-silencing effects of SYK and MSI1 on MB cell growth and MYC expression. HD-MB03 and D-283 cell lines were transfected with control (NTC), SYK and MSI1 siRNAs (each at 50 nM and 100 nM) for 72 h and subjected to (A) western blot (for the expression of SYK or MSI1 and MYC proteins) and (B) MTT (cell growth) analyses. GAPDH was used as the loading control in western blot analysis. Results are the representative of three independent experiments. Plotted values and error bars represent mean ± SEM. *p < 0.05 (Student-t-test)