Fig. 6

OTUD4 enhances radiosensitivity in NPC cells by promoting GSDME-dependent pyroptosis. A Western blotting showed the protein levels of OTUD4 and GSDME in HONE1 and 5-8F cells transfected with or without OTUD4 and shGSDME. B-G The above cells were exposed to the indicated irradiation dose, and then (B) phase-contrast cell imaging, (C) LDH release assay, (D) live cell imaging, (E) Annexin/PI assay, and (F) colony formation assay was performed at designated time points. G The dose-survival curves of the above cells are indicated. All data are presented as the mean ± SD of three independent experiments. H Representative bioluminescence images of tumors were taken on day 42. I Statistical analysis of photon flux (n = 5). J Tumor size was monitored every 4 days (n = 5). K Images of resected tumors. L Tumor weight on day 42 (n = 5). M Serum LDH concentrations were determined pre-radiotherapy and following the third and sixth radiotherapy treatments (n = 3). H Representative images of IHC staining of the tumor sections are shown. Scale bars represent 50 μm. ns, No significant difference. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001