Fig. 2

ELF3-AS1 and ELF3 are transcriptionally inhibited by both SNAI2 and SNAI1 in GC. A The transcripts abundance of ELF3-AS1 and ELF3 in SNAI2/SNAI1 overexpression cell lines was detected by RNA-seq. B The normalized expression (FPKM value) of SNAI1, SNAI2, ELF3 and ELF3-AS1 were shown in the plot. C The overexpression efficiency of SNAI1 in GC cell lines was verified by qRT-PCR. D, E The ELF3-AS1 and ELF3 expression were detected after overexpression of SNAI1. F Overexpression of SNAI1 or SNAI2 significantly decreased the protein level of ELF3. G Dual-luciferase reporter assay showed that the predictive binding site of SNAI1 or SNAI2 is necessary for their inhibition on Luc expression. H Diagram showing the primers location used in CHIP-qPCR/PCR. I, J Chip assay showed that both SNAI1 and SNAI2 could bind to the promoter of ELF3 or ELF3-AS1. **, P < 0.01