Fig. 4

ELF3 negatively regulates cell metastasis but cannot regulate ELF3-AS1 expression in GC. A-D ELF3-AS1 and ELF3 were highly co-expressed in GC cell lines and tissues. E ELF3-AS1 and ELF3 were highly co-expressed in human normal pan-tissues. The expression data of ELF3-AS1 and ELF3 were obtained from the NCBI web tool. F The transcripts abundance of ELF3-AS1 and ELF3 was detected by RNA-seq in ELF3-AS1-depleted cell lines. G The knockdown efficiency of ELF3-AS1 in GC cell lines was verified. H, I Knockdown of ELF3-AS1 decreased the mRNA and protein levels of ELF3 in GC. J, K The ELF3 knockdown/overexpression had no obvious effect on ELF3-AS1 expression. L, M ELF3 negatively regulates the migration and invasion of GC cells. **, P < 0.01