Fig. 5

ELF3-AS1 mainly inhibits GC metastasis through repressing SNAI2 signaling. A The heat map reveals the differential expressed miRNAs altered by ELF3-AS1 knockdown (left panel), and the log2FC values (ELF3-AS1/NC) were shown in the right panel. The red asterisk indicates the miRNAs targeting SNAI2. B The binding sites of miR-33a/b and miR-203a on 3’UTR of SNAI2 were predicted by the TargetScan web tool. SNAI2 mRNA was significantly decreased after overexpression of miR-33a, miR-33b or miR-203a, respectively. C-E The expression level of miR-33a, miR-33b and miR-203a were detected in the ELF3-AS1 knockdown cell lines. F The transcripts abundance of SNAI2 in ELF3-AS1 knockdown cell lines. G, H The mRNA and protein level of SNAI2 were determined in the ELF3-AS1-depleted GC cell lines. I Knockdown of ELF3-AS1 resulted in upregulation of SNAI2 mRNA in A549 and Hela cell lines. The expression levels of SNAI2 and ELF3-AS1 in GSE92250 were normalized to FPKM values. J, K Rescue assay in vitro and in vivo confirmed that ELF3-AS1 inhibited GC metastasis through SNAI2 signaling. Scale bars=50μm **P < 0.01