Fig. 7

The MDHDH locus was epigenetically silenced by PRC2/H3K28me3 in glioblastoma multiforme. A ChIP-seq peak tracks of H3K27me3 from the Cistrome Data browser (T98G cell line, CistromeDB: 103264, normal brain tissue CistromeDB: 6651) in the MDHDH loci. B Pearson’s correlation of the CGGA primary glioma (WHO grade I-IV) expression profiles between EZH2 and LINC00632. C Graphic representation of the relative MDH2 expression level (TPM) in different tissues (GBM vs. GTEx, LGG vs. GTEx) (*, statistically significant). D Representative immunoblot of H3K27me3 and total histone H3 in EZH2 inhibitor-treated U87 and U251 cells. (E). Normalized abundance (%ChIP/Input, qPCR) of the TSS region from mouse IgG, H3, or H3K27me3 ChIP samples isolated from DMSO- or EZH2 inhibitor-treated (GSK126, 5 μM for 6 days) U87 and U251 cells. Mean ± SD of a representative experiment. F Relative expression of LINC00632 transcript variant 3 (MDHDH) in U87 and U251 cells treated with GSK126 (5 μM) for the indicated number of days. Data were normalized to the DMSO-treated control (first time point). Mean ± SD of a representative time course