Fig. 7

CEBPA-DT induces EMT in hepatoma cells. A The expression levels of EMT markers were measured by RT-qPCR in the indicated HCCLM9 and Huh7 cells. B Relative luciferase activities of Snail1 promotor were examined by luciferase reporter assays in the indicated HCCLM9 and Huh7 cells. C The expression levels of DDR2 and EMT-related proteins Snail, E-cadherin, N-cadherin and Vimentin were measured by western blot assays. D The expression levels of EMT-related proteins Snail, E-cadherin and Vimentin were measured by IF assays. Scale bar, 50 μm. E The expression levels of EMT-related proteins Snail, E-cadherin, N-cadherin and Vimentin were detected by western blot assays in the indicated cells co-transfected with siSnail1 or negative control. Date are presented as mean ± SD; n = 3. Student’s t test was used. *p < 0.05, **p < 0.01, ***p < 0.001