Fig. 8

CEBPA-DT promotes the EMT process via the interaction between DDR2 and β-catenin. A The expression levels of β-catenin in the nuclear and cytosol in the indicated HCCLM9 and Huh7 were measured by the subcellular fractionation assays, β-actin and H3 were used as the cytoplasmic and nuclear endogenous control. B IF assays demonstrated that CEBPA-DT increased the nuclear translocation of β-catenin. Nuclei were stained with DAPI. Scale bar, 50 μm. C Schematic diagram of the predicted binding sites of β-catenin on the promoter region of Snail1 gene. D Left: ChIP assays showed the enrichment of β-catenin on BSs in the promoter region of Snail1 relative to IgG. Right: ChIP assays showed the enrichment of β-catenin on BSs in the Snail1 promoter region in the indicated cells. E TOP/FOP Flash assays showed the transcriptional activities of β-catenin signaling in the indicated HCCLM9 cells. F Co-IP assays showed CEBPA-DT regulated the interaction between β-catenin and DDR2 in the indicated cells. G IF assays showed CEBPA-DT mediated β-catenin nuclear redistribution was rescued in indicated Huh7 cells treated with DDR2-IN-1. H Western blot assays showed CEBPA-DT induced-EMT was regulated by DDR2-β-catenin axis. Date are presented as mean ± SD; n = 3. Student’s t test was used. *p < 0.05, **p < 0.01, ***p < 0.001