Fig. 1

MNX1-AS1 is a c-Myc associated pan-cancer lncRNA. a Volcano Plot showing differentially expressed lncRNAs based on RNA-seq analysis of P493–6 cells carrying a Tet-Off c-Myc expression system following the induction of c-Myc with 1 μM doxycycline. MNX1-AS1 is highlighted in blue. b Venn diagram showing the intersection of c-Myc-upregulated lncRNAs from (A) versus pan-cancer lncRNAs identified from the TCGA database. c qPCR assays measuring MNX1-AS1 expression in hepatocellular carcinoma and normal cell lines. d Absolute quantitation of MNX1-AS1 by digital PCR in hepatocellular carcinoma and normal cell lines. e Northern blotting against MNX1-AS1 comparing HepG2 cells bearing sh-ctrl or sh-MNX1-AS1 lentiviruses. f, g Fractionation of HepG2 cells to determine the subcellular localization of MNX1-AS1. Western blotting against control proteins PARP and GAPDH (F) and qPCR against MNX1-AS1, β-actin and U1 control RNAs (G) in cytoplasmic and nuclear fractions, respectively. h Fluorescence in situ hybridization in HepG2 cells against MNX1-AS1 (red) with cell nuclei decorated by DAPI (blue). C and D are mean ± SD; n = 3 independent experiments, one-way ANOVA with Tukey’s multiple comparison post-test, *p < 0.05, ***p < 0.001. G is mean ± SD; n = 3 independent experiments, two-tailed paired Student’s t test, ***p < 0.001. E, F and H are represent of three independent experiments