Fig. 1

Expression and validation of circRUNX1 in ESCC tissues and cells. A Expression of top eight circRNAs in 25 pairs of ESCC tissues was detected by qRT-PCR. B qRT-PCR analysis of circRUNX1 expression in 54 pairs of ESCC tissues (25 pairs in panel A and another 29 pairs of ESCC tissues). C Diagnosed value of circRUNX1 was displayed in ROC curve. D Relative expression of circRUNX1 in a panel of ESCC cell lines and normal esophageal epithelial cells (HEEC). E Schematic illustration of circRUNX1 formation via the circularization from exon 5 to exon 6 in the RUNX1 gene located on chromosome 21. F The expression of circRUNX1 and RUNX1 mRNAs after treatment with RNase R in KYSE150 cells. G Convergent and divergent primers were used to verify closed loop structure of circRUNX1. H The expression changes of circRUNX1 (cRUNX1) and RUNX1 (mRUNX1) were detected by qRT-PCR in KYSE150 and TE1 cells treated with actinomycin D at the indicated time points. I The abundance of circRUNX1 in the nuclear and cytoplasmic of KESE150 and TE1 cells was measured by qRT-PCR. J FISH assays for circRUNX1 in KESE150 and TE1 cells. Nuclei were stained with DAPI and cytoplasm was staind with 18S. (Magnification × 400. Scale bar: 20 μm and Magnification × 1000. Scale bar: 10 μm). The data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001