Fig. 1
NAT10 and the level of ac4C modification are frequently upregulated in CRC. A The mRNA level of NAT10 detected by qRT-PCR in 80 CRC tissues and matched adjacent tissues. B-D The protein level of NAT10 detected by IHC in the TMA of 80 CRC patients’ samples. E and F The level of ac4C modification detected by IHC in the TMA. G The detection of the protein level of NAT10 by WB in 8 matched CRC tumor and adjacent tissues. H Level of ac.4C modification in 8 paired CRC tumor and adjacent tissues, as determined by dot blot. I-K The mRNA and protein levels of NAT10 and the level of ac4C in CRC cell lines and NCM460 cells, as determined by qRT-PCR, WB and dot blot respectively. Data are shown as mean ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001