Fig. 4
Identification of the NAT10 targets in CRC cells. A Heatmap of significantly down-regulated genes in NAT10 knockdown cells identified by RNA-seq. B GO enrichment analysis of the significantly down-regulated genes. C Distribution of NAT10 targeted transcripts identified by RIP-seq and acRIP-seq. D The distribution of NAT10-binding regions and ac4C peaks across mRNA in CRC cells. E The consensus sequences of NAT10-binding sites and ac4C motif were detected by the motif analysis with RIP-seq and acRIP-seq data. F GO enrichment analysis of the genes overlapped from the genes identified by RIP-seq and acRIP-seq. G Overlapping analysis of genes identified by RNA-seq, RIP-seq, and acRIP-seq. H. The list of the potential direct targets of NAT10. I Distribution of NAT10-binding regions and ac4C peaks on KIF23 mRNA visualized by IGV