Fig. 4

miR-214 is transferred from stroma to tumor cells. A-D miR-214 and miR-223 expression levels in Extracellular Vesicles (EVs) derived from miR-214^over and miR-214^wt MEFs or from HS5 were previously transduced with a miR-214sponge (miR-214^sponge) or an empty (control) expressing vector, measured by qRT-PCR analysis. E–F miR-214 expression levels in B16-F10 and MA-2 cells pretreated for 24 h with EVs derived from miR-214^over or miR-214^wt MEFs or from miR-214 sponged HS5 (miR-214^sponge) or empty controls (control), measured by qRT-PCR analysis. A-F Results are shown as fold changes (mean ± SD of triplicates) relative to controls, normalized on U6. At least 2 independent experiments (with triplicates) were performed and representative results are shown. G-H Expression levels of TFAP2C, ALCAM and ITGA5 in B16-F10 (G) and MA-2 (H) cells pretreated for 24/48 h with EVs derived from miR-214^over and miR-214^wt MEFs (G) or from HS5 (H) miR-214sponged HS5 (miR-214^sponge) or empty controls (control), measured by Western Blot analysis. Protein modulations were calculated relative to controls, normalized on the loading control and expressed as percentages. MEFs = murine embryo fibroblasts; SEM = standard error of mean. SD = standard deviation. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001