Fig. 2

Colon cancer cells promoted the proliferation and migration of Schwann cells by stimulating their secretion of NGF. A. The proliferation of Schwan cells co-cultured with FHC, SW480, and HCT116 cells or not was assessed via CCK8 for 3 days. B. The expression of p27kip1, CyclinD1, and CyclinE of Schwann cells co-cultured with FHC, SW480, and HCT116 cells or not was detected by Western blot and qRT-PCR. C. The wound healing assays showed that co-cultured with colon cancer cells significantly augmented the migrative ability of Schwann cells. D. The transwell assays indicated that co-cultured with colon cancer cells increased the migrative abilities of Schwann cells. E, F. The expression of NGF in Schwann cells was increased when co-cultured with colon cancer cells by Western blot and qRT-PCR. G. ELISA assay showed the expression of NGF in Schwann cells was augmented when co-cultured with colon cancer cells. H. CCK8 assay showed that inhibition of NGF reversed the enhanced proliferation of Schwann cells co-cultured with SW480 cells. I. Western blot and qRT-PCR showed that inhibition of NGF blocked the altered expression of p27kip1, CyclinD1, and CyclinE in Schwann cells that co-cultured with SW480 cells. Ro 08-2750 :the inhibitor of NGF. All data were revealed as means ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ***P < 0.001. n.s means the difference was not significant