Fig. 6

Schwann cells facilitated the proliferation, migration, invasion, and EMT of colon cancer cells through NGF. A. CCK8 assay showed that inhibition of NGF reversed the enhanced proliferation of SW480 cells co-cultured with Schwann cells. B. Colony formation assay indicated that inhibition of NGF reversed the increased number and size of the colony co-cultured with Schwann cells. C. Western blot and qRT-PCR showed that inhibition of NGF blocked the altered expression of p27kip1, CyclinD1, and CyclinE in SW480 cells that co-cultured with Schwann cells. D. The wound healing assays indicated that inhibition of NGF significantly reversed the strengthened migrative ability of SW480 cells co-cultured with Schwann cells. E. The transwell assays indicated that inhibition of NGF reversed the strengthened migrative ability of SW480 cells co-cultured with Schwann cells. F. Western blot and qRT-PCR showed that inhibition of NGF blocked the altered expression of Vimentin, N-cadherin, E-cadherin, and ZO1 in SW480 cells co-cultured with Schwann cells. Ro: Ro 08-2750, the inhibitor of NGF. All data were revealed as means ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ***P < 0.001. n.s means the difference was not significant