Fig. 7

NGF modulated the proliferation and metastasis of colon cancer cells by TrkA. A, B. The expression of TrkA, phosphorylated TrkA, and p75 in colon cancer cells upon administration of NGF were detected by Western blot and qRT-PCR. C. CCK8 assay showed that inhibition of TrkA reversed the enhanced proliferation of SW480 cells upon administration of NGF. D. Colony formation assay indicated that inhibition of TrkA reversed the increased number and size of the colony upon administration of NGF. E. Western blot and qRT-PCR showed that inhibition of TrkA blocked the altered expression of p27kip1, CyclinD1, and CyclinE in SW480 cells caused by NGF. F. The wound healing assays indicated that inhibition of TrkA significantly reversed the strengthened migrative ability of SW480 cells upon administration of NGF. G. The transwell assays indicated that inhibition of TrkA reversed the strengthened migrative ability of SW480 cells upon administration of NGF. H. Western blot and qRT-PCR showed that inhibition of TrkA blocked the altered expression of ZEB1, Vimentin, N-cadherin, E-cadherin, and ZO1 upon administration of NGF. DMSO was used as a control. GNF5837: the inhibitor of TrkA. All data were revealed as means ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ***P < 0.001. n.s means the difference was not significant