Fig. 9

Schwann cells mutually increased miR-21-5p expression by regulating ZEB1. A. The qRT-PCR assays showed the expression of miR-21-5p in SW480 cells and HCT116 cells when co-cultured with Schwann cells. B. The qRT-PCR assays showed the expression of miR-21-5p in exosomes of SW480 cells and HCT116 cells when co-cultured with Schwann cells. C, D. The qRT-PCR assays showed the expression of intracellular and exosomal miR-21-5p in colon cancer cells when knockdown or overexpression of ZEB1. E, F. The qRT-PCR assays showed that knockdown of ZEB1 blocked the upregulated miR-21-5p expression in cells and exosomes of colon cancer when co-cultured with Schwann cells. ZEB1-OE:pcDNA3.1-ZEB1. All data were revealed as means ± standard deviation (SD) for no less than three independent experiments. Significant P values showed as ***P < 0.001