Fig. 4
From: SMAD9-MYCN positive feedback loop represents a unique dependency for MYCN-amplified neuroblastoma
SMAD9 knockdown inhibits NB growth in vivo. A Photographs of nude mice subcutaneously xenografted with BE(2)-C cells that had been stably transfected with Tet-on-shSMAD9 constructs. B, C Tumor mass (B) and tumor volume growth curve (C) in xenografted mice. D SMAD9 knockdown in shSMAD9–1# and shSMAD9–3# of Dox-off and Dox-on mice. E Representative images of HE and IHC (Ki-67 for proliferation and c-Cas3 for apoptosis) staining (40x magnification) in tumor tissue from shSMAD9 Dox-off and Dox-on mice. F Quantification of the percentage of Ki-67 and c-Cas3 positive cells. c-Cas3: cleaved caspase 3; Dox: doxycycline; HE: hematoxylin and eosin; IHC: immunohistochemistry. * P < 0.05, **P < 0.01, and *** P < 0.001