Fig. 5

A Correlations among HIF1α, FASN, and SLC7A11 based on the TCGA database. B and C HIF1α protein expression in FASN-knockdown and control Huh7SR and 7721SR cells. D Sublocalization of FASN and HIF1α in the cytoplasm in Huh7SR and 7721SR cells under normoxic and hypoxic conditions as determined by IF analysis. E Detection of binding between FASN and HIF1α by immunoprecipitation under normoxic and hypoxic conditions. F Schematic representation of the HIF1α recognition motif obtained from the JASPAR database (top panel) and three fragments of the SLC7A11 promoter sequence (bottom panel). G The pGL4.10- SLC7A11 promoter reporter gene was co-expressed with HIF1α in HEK293T cells for 24 h. Luciferase expression was measured and normalized to Renilla. H Schematic diagram of HIF1α binding site of the SLC7A11 promoter and the binding sequence. I and J HIF1α and SLC7A11 protein expression in HIF1α-overexpression and control Huh7SR and 7721SR cells. K and L Effect of YC-1 on FASN, HIF1α and SLC7A11 protein expression in Huh7SR and 7721SR cells overexpressing FASN. *p < 0.05; **p < 0.01; ***p < 0.001. sora, sorafenib; Z-VAD, Z-VAD-FMK; Ferro1, ferrostatin-1; Necro-1, necrostatin-1