Fig. 6

STRIP2 interacts with IGF2BP3. a Silver stained gel shows differential bands between control and STRIP2 samples. Red box indicates differential bands. b Tandem mass spectrum analysis of target band. c Interaction between STRIP2 and endogenous IGF2BP3 was demonstrated using immunoprecipitation. PC9 cells were transfected with Flag-STRIP2. d Interaction between endogenous STRIP2 and IGF2BP3 was demonstrated using immunoprecipitation. A549 cells were transfected with IGF2BP3-myc. e Interaction between STRIP2 and IGF2BP3 was demonstrated using immunoprecipitation. 293 T cells were concurrently transfected with Flag-STRIP2 and IGF2BP3-myc. f In vitro interaction between STRIP2 and IGF2BP3 was conducted by GST-pulldown assay. GST protein was identified using Coomassie staining. g The domain of STRIP2 (339–834) was interacted with IGF2BP3 using immunoprecipitation. h The domain of IGF2BP3 (199–407) was interacted with STRIP2 using immunoprecipitation