Fig. 1
Cell populations with a fibroblastoid morphology isolated from PM patients lack chromosomal abnormalities and express markers of cancer-associated fibroblasts. A Micrographs of cultured Meso109F and the biphasic PM cell line SPC212 were taken at low and high (insets) density. Scale bar = 100 μm. B Doubling times (mean and SEM) for the Meso-CAFs, the sarcomatoid PM cell line Meso84, the biphasic PM cell lines SPC212 and MSTO-211H, and the epithelioid PM cell line VMC23 were calculated as described in Materials and Methods. C Array comparative genomic hybridization data for Meso109F and SPC212. Gains and losses of gene dose across all chromosomes compared to the reference DNA are depicted as elevations and depressions of the horizontal line, respectively. D The indicated cell lines were subjected to qRT-PCR to determine the expression of genes previously described as fibroblast or PM marker genes. Each dot represents the mean of at least three biological replicates. Gene expression data were normalized to GAPDH and β-Actin expression and are shown as 2–ΔCt * 105